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Magnesium Inhibits Wnt/β-Catenin Activity and Reverses the Osteogenic Transformation of Vascular Smooth Muscle Cells

Figure 1

The effect of magnesium on calcification and osteoblast-like transformation in VSMC in vitro.

VSMC were cultured with 3.3 mM P, and increasing concentrations of magnesium (0.8 mM/1.4 mM/2.6 mM); control group with 0.9 mM P+0.8 mM Mg was also included. (A), calcium content of VSMC cultured for 9 days. Relative mRNA expression of Cbfa-1 (B) and osterix (C); a, P<0.001 vs Control; b, P<0.001 vs VSMC with 3.3 mM P+0.8 mM Mg. Bars are the mean±SE of three experiments in quadruplicate.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0089525.g001