Increasing Prion Propensity by Hydrophobic Insertion
Figure 2
In vitro amyloid aggregation of the mutant prion forming domains.
Aggregation of purified PFDs was monitored using thioflavin T. Reactions were incubated with intermittent shaking for 48 h. Fluorescent readings were taken approximately every 90 min. Error bars represent the standard deviations of three samples.