The Redox Function of APE1 Is Involved in the Differentiation Process of Stem Cells toward a Neuronal Cell Fate
Figure 5
Effects of E3330 on cell viability, proliferation and APE1 expression.
(A) Histograms summarizing the results of a viability assay (MTT) performed following the exposure of undifferentiated hAT-MASC to either positive control (DMSO), negative control (untreated), or 10 µM, 25 µM, 50 µM, 75 µM, and 100 µM of E3330. (B) Histograms summarizing the effects of the exposure of hAT-MASC to E3330 -during either the last step (N3) or the last two steps (N2N3) of differentiation- on the yield of cells at the end of the differentiation protocol. (C) Immunofluorescence images showing the effects the exposure of hAT-MASC to E3330–during either N3 or N2N3- on APE1 expression (green). Nuclei are blue labeled by DAPI. (D) Quantification of Ape1 nuclear expression (IFI) is summarized in histograms. *, **, ***, ***** p<0.05 vs I, II, III, and V column, respectively.