Selective Activation of hTRPV1 by N-Geranyl Cyclopropylcarboxamide, an Amiloride-Insensitive Salt Taste Enhancer
Figure 5
Effects of NGCC in amiloride-sensitive ASIC1a.
(A) Rapid change of extracellular pH from 7.4 to 6.0 for 10 seconds induced an inward current in HEK293T cells transiently transfected with mASIC1a. Current density of pH 6.0-evoked inward currents is 5.52±1.33 pA/pF (n = 3) and 118.1±33.0 pA/pF (n = 3) in non-transfected cells and mASIC1a-expressing cells, respectively. (B) mASIC1a currents were triggered by extracellular pH drop from 7.4 to 6.0. However, ASIC1a channels are not sensitive to NGCC (1 mM). The time interval between each stimulation is 120 seconds. Normalized current density was measured in cells expressing mASIC1a (n = 5). Results are presented as the mean ± SEM.