Analysis of Poly(ADP-Ribose) Polymerases in Arabidopsis Telomere Biology
Figure 7
PARPs are not required to maintain telomere length in Arabidopsis.
(A) TRF analysis of bulk telomeres in several individual wild type, parp1, parp2, and parp1 parp2 plants. (B–D) PETRA analysis of telomeres on specific chromosome arms in wild type and parp mutants. Primer naming convention is the same as in the Figure 6 legend. (B) Analysis of the telomeres on chromosome arms 1L and 2R. DNA was from pooled seedlings. (C) Analysis of four different chromosome arms for individual parp1, parp2, and parp1 parp2 mutant plants. Chromosome arms tested are listed below the lanes. (D) Analysis of wild type and G4 tert pooled seedlings grown in either 0.6% DMSO or 5 mM 3-AB/0.6% DMSO using the 2R primer. Molecular weight markers are shown to the left of each gel.