Temsirolimus Inhibits Proliferation and Migration in Retinal Pigment Epithelial and Endothelial Cells via mTOR Inhibition and Decreases VEGF and PDGF Expression
Figure 8
Protein levels of PDGF in HUVEC and pRPE cell supernatants (ELISA).
Protein levels of PDGF were measured in a similar manner as VEGF expression from supernatants with an ELISA assay. Cells were exposed to 0.05 µg/ml temsirolimus for 24 h. A significant decrease in PDGF protein expression for both HUVEC (A) and RPE cells (B) was detected. Each value was normalized to a standard curve of PDGF, and normalized to the untreated control of each cell line. Data are expressed as means ± SD. (*p<0.05).