Temsirolimus Inhibits Proliferation and Migration in Retinal Pigment Epithelial and Endothelial Cells via mTOR Inhibition and Decreases VEGF and PDGF Expression
Figure 3
Migration of HUVEC and pRPE cells.
HUVEC and RPE cell migration was significantly inhibited after exposure to temsirolimus in a dose-dependent manner (µg/mL) assessed by a modified Boyden chamber method. A) Example of cells attached to a permeable membrane at different concentrations compared to an untreated control. B) Evaluation of migrated HUVEC in percent control after treatment with three different temsirolimus concentrations. C) Migrated RPE cells compared to untreated cells in percent control. Data are expressed as means ± SD with *p<0.05.