Lactate Regulates Rat Male Germ Cell Function through Reactive Oxygen Species
Figure 5
(A) and (B) Mechanisms involved in lactate regulation of ROS levels in male germ cells.
Male germ cells were incubated for 30α-cyano-4-hydroxy-cinnamate (CHC) 10 mM, oxamate (OXA) 10 mM (A), apocynin (APO) 500 µM, allopurinol (ALL) 100 µM or rotenone (ROT) 1 µM (B). Cell extracts were prepared and utilized for TBARS assay. Values are expressed as means±S.D. of triplicate incubations in one representative experiment out of three. Different letters indicate statistically significant differences among treatment groups (p<0.05). (C) Expression of NOX family members in male germ cells. Total RNA of rat colon (C), skeletal muscle (M), kidney (K), testis (T), Sertoli cells isolated as previously described [44] (SC) and male germ cells (GC) were extracted and analyzed by RT-PCR and visualized by ethidium bromide staining. NT is no template control.