Lactate Regulates Rat Male Germ Cell Function through Reactive Oxygen Species
Figure 2
Effect of lactate on ROS production in male germ cells.
Male germ cells were incubated for variable periods of time (15, 30 or 60 minutes) in the absence or presence of lactate 20 mM or H2O2 500 µM (positive control) (A), or incubated for 30 minutes in the absence or presence of different doses of lactate (10 or 20 mM) (B). Cell extracts were prepared at the designated intervals and utilized for TBARS assay. Values are expressed as means±S.D. of triplicate incubations in one representative experiment out of three. *P<0.05 versus basal (B).