A Pre- and Co-Knockdown of RNAseT Enzyme, Eri-1, Enhances the Efficiency of RNAi Induced Gene Silencing in Caenorhabditis elegans
Figure 2
Effect of different employed RNAi methodology on dumpy phenotype in control N2 strain (A), RNAi induced gene silencing of dpy-9 gene (B), dpy-9 RNAi clone co-incubated with eri-1 RNAi clone (C), dpy-9 RNAi clone co-incubated with lin-35 RNAi clone (D) dpy-9 RNAi clone co-incubated with eri-1 and lin-35 RNAi clones (E), Pre-incubation with eri-1 RNAi clone followed by incubation with dpy-9 RNAi clone (F) Pre-incubation with eri-1 RNAi clone followed by co-incubation with dpy-9 and eri-1 RNAi clones(G),Pre-incubation with eri-1 RNAi clone followed by co-incubation with dpy-9, eri-1 and lin-35 RNAi clones(H), Pre-incubation with dpy-9 RNAi clone followed by incubation with eri-1 RNAi clone (I) Pre-incubation with dpy-9 RNAi clone followed by co-incubation with dpy-9 and eri-1 RNAi clones (J) Pre-incubation with dpy-9 RNAi clone followed by co-incubation with dpy-9, eri-1 and lin-35 RNAi clones (K).
Fig 1L is a graphical presentation of worms showing percentages of dumpy phenotypes examined by three different RNAi methodologies. *p<0.05, NS - Not significant.