Transcriptome Analysis in Venom Gland of the Predatory Giant Ant Dinoponera quadriceps: Insights into the Polypeptide Toxin Arsenal of Hymenopterans
Figure 9
Multiple alignments of deduced amino acid sequences of different identified dinoponeratoxins from the D. quadriceps venom gland transcriptome and D. australis.
Deduced D. quadriceps dinoponeratoxin cDNA precursor sequences (contig_1 and contig_9). RNA deep sequencing contigs (contig_2 and contig_145) were compared to mature peptide sequences from D. australis (Da-3177 and Da-3105) (part A) and from another species of Dinoponera (Da-2501) (part B). ClustalW was used to multi-align the sequences. Identical amino acid residues are marked with asterisks. Stretches of deduced amino acid sequences supported by EST sequences are boxed. Signal peptides (pre-peptides) are doubled underlined, and pro-regions of pro-peptides are shown with a single line under the sequence. Contigs 1 and 9 were first identified in the EST library, and contigs 2 and 145 came primarily from RNA-Seq.