Transcriptome Analysis in Venom Gland of the Predatory Giant Ant Dinoponera quadriceps: Insights into the Polypeptide Toxin Arsenal of Hymenopterans
Figure 2
Overall classification of ESTs from the D. quadriceps venom gland cDNA (ESTs) library based on function.
EST sequences were annotated by comparing contigs and singlets (E-values≤1.0E-5) with the non-redundant Genbank translated protein database using BLASTX. Search stringency was eventually adjusted (E-values lower than 1.0E-5) and database restricted to hymenopterans with the aim of finding relevant familial- and structural-related sequences of hymenopterans toxins. Part A – relative number of polypeptides which were counted as individual random sequenced clones. The group of <cellular process> refers to enzymes and polypeptides involved in the venom gland metabolism. Part B – relative numbers of venom-related sequences found in the overrepresented small toxin dataset. Under the term <toxin> is included dinoponeratoxins, venom allergens, and venom cysteine rich peptides (part B).