Activation of Nlrp3 Inflammasomes Enhances Macrophage Lipid-Deposition and Migration: Implication of a Novel Role of Inflammasome in Atherogenesis
Figure 5
Increased lipid accumulation in BMMs with activation of Nlrp3 inflammasomes by ATP.
BMMs were primed with LPS (1 ng/ml) for 3 h and treated with ATP (2.5 mM, 16 h), IL1β (0.5 ng/ml), MSU (100 µg/ml), or IL18 (25 nM). Some group of BMMs were treated with ATP or MSU in the presence of caspase-1 inhibitor WEHD (0.15 µg/ml) or IL1R antagonist (IL1Ra, 40 ng/ml). Then, BMMs were loaded with oxLDL (10 µg/mL) for 16 hours. (A) Light microscopic images show oil red O stained BMMs. Cells were counterstatined with hematoxylin. (B) Summarized data showing area percentage of each cell positive for Oil red O staining in BMMs. * P<0.05 vs. untreated Asc+/+ control group; # P<0.05 vs. Asc+/+ ATP group; $ P<0.05 vs. Asc+/+ MSU group (n = 6).