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Activation of Nlrp3 Inflammasomes Enhances Macrophage Lipid-Deposition and Migration: Implication of a Novel Role of Inflammasome in Atherogenesis

Figure 1

ATP, cholesterol crystal or 7-ketocholesterol increased inflammasome formation in Asc+/+ BMMs.

(A) Representative confocal fluorescence images and summarized data depicting the effect of ATP (0–7.5 mM, 16 h) on the colocalization between Nlrp3 and Asc or caspase-1 in Asc+/+ BMMs. (B) Representative confocal fluorescence images and summarized data depicting the effect of cholesterol crystal (0–1 mg/ml, 16 h) on the colocalization between Nlrp3 and Asc or caspase-1 in Asc+/+ BMMs. (C) Representative confocal fluorescence images and summarized data depicting the effect of 7-ketocholesterol (0–20 µg/ml, 16 h) on the colocalization between Nlrp3 and Asc or caspase-1 in Asc+/+ BMMs. * P<0.05 vs. untreated control group (n = 6).

Figure 1

doi: https://doi.org/10.1371/journal.pone.0087552.g001