Chitosan Scaffolds Containing Calcium Phosphate Salts and rhBMP-2: In Vitro and In Vivo Testing for Bone Tissue Regeneration
Figure 5
Surgery (A), gross morphology after euthanasia (B, C) and microCT analysis of samples (D).
Surgery images (A) show scaffold implantation process. After euthanasia gross morphology images were obtained at different angles (B,C) (Dotted circle indicates defect location). Defect area is still observed in CPS-CHI implanted tibias (B), while in rhBMP2-CPS-CHI implanted tibias high amount of newly-formed hard tissue, apparently bone, appeared vertically from the defect (C). MicroCT study (D) confirms trabecular bone formation in rhBMP2-CPS-CHI implanted tibias, while it seems no scaffold-resorption in any case. Neither seems a robust new bone formation in the rest of implanted scaffolds compared to empty controls.