Specific Expression of DR5 Promoter in Rice Roots Using a tCUP Derived Promoter-Reporter System
Figure 3
Comparison of GUS leaky expression in calli among vectors with different selectable marker promoters in different stacking configurations.
(A) Distribution histograms showing the numbers of spots of GUS leaky expression in calli transformed by group A (a1–a3), C (c1–c3) and D (d1–d3) vectors. Numbers of GUS spots per callus was set in the range from 0 to 80 and divided into 9 intervals. A total of 50 calli were counted for each vector. (B) Average number of GUS spots per callus derived from each vector. The bar represents mean±SE of spot numbers from 50 calli counted in (A). Different letters above the column indicate a statistically significant difference (p<0.05) between vectors with the same selectable marker promoter. (C) Relative GUS leaky percentage between group A (a1–a3) and group C (c1–c3) vectors after normalization by average spot numbers per callus of the respective group D (d1–d3) vectors. Different letters above the column indicate a statistically significant difference (p<0.05) between vectors with the same configuration. Statistical analysis was performed with GraphPad Prism 6 software (GraphPad Software Inc., La Jolla, CA) by using one-way ANOVA with a Tukey’s multiple comparisons test.