Dominant Effects of the Diet on the Microbiome and the Local and Systemic Immune Response in Mice
Figure 5
Diet-mediated effects on a primary and secondary infection with C. rodentium.
(A) Shedding of C. rodentium in the feces following a primary infection (n = 14 mice/group). (B) Shedding of C. rodentium in the feces following a secondary infection (n = 14 mice/group). Values are the mean +/− SEM. TD was significantly different from PD (*P<0.05, **P<0.01, ***P<0.001, two-way ANOVA with Bonferroni post-tests). (C) Fecal DNA was collected from PD-fed (lanes 1–4) and TD-fed mice (lanes 5–8) prior to secondary infection and (D) cluster analysis of the similarity of the DGGE banding patterns from panel C. CFU, colony forming units, DGGE, denaturing gradient gel electrophoresis; PD, purified diet; TD, Teklad diet.