Antimicrobial Cathelicidin Peptide LL-37 Inhibits the LPS/ATP-Induced Pyroptosis of Macrophages by Dual Mechanism
Figure 5
Effect of LL-37 on the ATP-induced caspase-1 activation in J774 cells.
J774 cells were treated with 3-37 (0.01, 0.1 or 1 µg/ml), P2X7 antagonists (1 µM KN-62 and 1 µM KN-93) or dimethylsulfoxide (DMSO, a solvent for KN-62 and KN-93, 0.1%). Cells were also incubated without ATP, LL-37 and P2X7 antagonists (Resting). Thereafter, the caspase-1 activation was assayed by flow cytometry using FAM-YVAD-fmk (FLICA), and expressed as the percentage of FLICA positive cells. Data shows the mean ± SD of 3 separate experiments. Values are compared between the absence and presence of LL-37 or P2X7 antagonists among ATP-treated cells. **P<0.01, ***P<0.001.