Cell-Penetrating Peptide-Mediated Delivery of TALEN Proteins via Bioconjugation for Genome Engineering
Figure 2
TALEN conjugation is reversible and R9 must be removed for TALEN cleavage activity.
(A) Purified TALEN proteins are incubated with Cys-nitropyridyl (Npys) Arg9 cell-penetrating peptide (R9-CPP) for 1 hr at room temperature. (B) In vitro cleavage analysis of TALEN proteins conjugated at (left) various peptide-to-protein ratios and (right) various protein concentrations at a 30-to-1 peptide-to-protein ratio in the (top) absence or (bottom) presence of 10 mM DTT.