Genes Involved in Degradation of para-Nitrophenol Are Differentially Arranged in Form of Non-Contiguous Gene Clusters in Burkholderia sp. strain SJ98
Figure 4
Enzyme assay of p-nitrophenol 4-monooxygenase (PnpM)
(A) Spectrophotometric analysis PnpM activity as shown by decrease in spectral absorption at 420 and 340 nm corresponding to depletion of p-nitrophenol (PNP) and NADH in a time dependent manner. (B) Lack of PnpM activity in negative control sample (C) Enzymatic transformation of PNP to BQ was confirmed by HPLC analysis.