Genes Involved in Degradation of para-Nitrophenol Are Differentially Arranged in Form of Non-Contiguous Gene Clusters in Burkholderia sp. strain SJ98
Figure 3
Representative SDS PAGE gel picture of
(A) His-6-PnpA purification by Ni-NTA chromatography, Lane M) Marker, Lane 1) uninduced, 2) induced whole cell lysate, 3) Induced supernatant, 4) pellet, 5) & 6) flow through, 7) wash and lane 8) Elution of His-6-PnpA is showing with a red arrow.(B) His-6-PnpB purification by Ni-NTA chromatography, Lane M) Marker, Lane1) uninduced , 2) induced whole cell lysate, 3) Induced supernatant, 4) pellet, 5) & 6) flow through, 7) wash and lane 8) Elution of His-6-PnpB is showing with a red arrow.