Finasteride Inhibits Human Prostate Cancer Cell Invasion through MMP2 and MMP9 Downregulation
Figure 4
Finasteride’s effects on the migration of the human prostate cell lines RWPE-1, LNCaP, PC3 and DU145.
The prostate cell lines were previously cultivated in 50 µM finasteride or control medium for 72 hours and individually cultivated in 200 µl of serum-free medium (with or without 50 µM finasteride) in the migration insert at a density of 1×105 total cells. After 22 hours, cells that migrated through the 8 µm porous membrane were fixed, stained and counted within five random fields using a light microscope. Experiments were performed in triplicate. Representative photomicrographs of untreated and finasteride-treated cells are shown on the left side. Human fibroblasts (WS1 cell line) were employed as migration-positive control cells, and a representative image is shown on the bottom-left side. Scale bar = 40 µm. Finasteride significantly inhibited the cell migration of the RWPE-1 and LNCaP cell lines but not of the PC3 and DU145 cell lines. Data are expressed as the mean ± SD of the migrating cells. (*) Statistically significant values with p<0.05. (**) Statistically significant values with p<0.01.