Finasteride Inhibits Human Prostate Cancer Cell Invasion through MMP2 and MMP9 Downregulation
Figure 1
Finasteride’s effects on MMP2 and MMP9 activities in the conditioned medium of DU145 cells.
a) Conditioned medium (CM) of untreated (Control) and finasteride-treated DU145 cells was collected, concentrated and analyzed for MMP2 and MMP9 activities using a gelatin zymography assay. (S) Standards, (P) Tissue extract of a gastric ulcer that was used as a positive control. These cells secreted high quantities of MMP2 and MMP9 into their CM, as shown by the bright bands on the dark background. b) High-dose finasteride (50 µM) downregulated MMP2 activity up to 43% after 72 hours of exposure, as quantified using the ImageJ™ software. Data are expressed as a fold-change graphic of the IOD values that were obtained from the bands in the (a) figure for finasteride-treated cells over control cells. (**) Statistically significant values with p<0.01. Experiments were carried out in triplicate.