Antagonism of Bradykinin B2 Receptor Prevents Inflammatory Responses in Human Endothelial Cells by Quenching the NF-kB Pathway Activation
Figure 4
Effect of fasitibant on angiogenesis and circulating proangiogenic cells in experimental osteoarthritis in rats.
(A) Representative images of hematoxylin/eosin staining (20 X) in synovial tissue from rats after intra articular injection of: saline (a), MIA (b), fasitibant with saline (c) or MIA with fasitibant, as described in Materials and Methods. Bar = 100 µM. Inset at higher magnification (40 X). Bar = 50 µM. (B–C) Representative images and quantification of CD-31 staining (40 X) in rats as described in A; Bar = 50 µM; quantification of CD-31 was performed counting 10 random field/section for slides; each slide has four sections. Data represent vessels counted for section in synovial tissue **P<0.01 compared to saline; ##P<0.01 compared to MIA. (D) ELISA immunoassay for VEGF in synovial fluids of rats treated as described in A. ***P<0.001 compared to saline; ##P<0.01 to MIA. (E) Representative images of CD-133 staining in rats as described in A; Bar = 50 µM. Inset at higher magnification (40 X).