Antagonism of Bradykinin B2 Receptor Prevents Inflammatory Responses in Human Endothelial Cells by Quenching the NF-kB Pathway Activation
Figure 1
B2R stimulation promotes changes of cell permeability.
(A) mRNA expression for B1 and B2 receptors, (B2R mRNA is approximately 339 pb) and (B) western blot analysis of B2 receptor in HUVEC. (Experiments are run three time; n = 3). (C) Permeability in HUVEC monolayer was detected as passage of fluorescence-coniugated FITC-Dextran from upper to lower compartments (Numbers represent mean ± SEM of three experiments run in triplicate; n = 3); ***p<0.001, **p<0.01, *p<0.05 compared to untreated cells (D). Fasitibant (1 µM), prevents the enhanced permeability, n = 3; ***p<0.001 compared to untreated cells #p<0.05, ###p<0.001 to BK-treated cells.