Biosynthesis of Firefly Luciferin in Adult Lantern: Decarboxylation of ʟ-Cysteine is a Key Step for Benzothiazole Ring Formation in Firefly Luciferin Synthesis
Figure 10
Identification of arbutin in L. lateralis by HPLC analysis.
A. HPLC analysis of the extracts from an adult L. lateralis by using a fluorescence detector. (a) authentic arbutin, (b) the extracts of L. lateralis lantern. The arbutin fraction between the vertical dashed lines is used for hydrolysis as in Fig. 10C. B. HPLC analysis of the hydrolyzed arbutin fraction in Fig. 10A–b. (a) authentic 1,4-hydroquinone (labeled peak 1) containing benzoquinone (labeled peak 2), (b) the hydrolyzed products of the peak fraction between the dashed lines in Fig. 10A–b. Asterisk indicates 1,4-hydroquinone from arbutin. C. A scheme of acid hydrolysis of arbutin to 1,4-hydroquinone by acid treatment with HCl.