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Agrobacterium tumefaciens-Mediated Transformation of the Lichen Fungus, Umbilicaria muehlenbergii

Figure 2

Map of binary vector pSK1044.

pBHt2 contains the hygromycin B resistance gene under the control of the Aspergillus nidulans trpC promoter [8] was used to construct pSK1044 by insertion of the eGFP gene cassette between the EcoRI and HindIII sites in the multi-cloning site of pBHt2.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0083896.g002