Gambogenic Acid Kills Lung Cancer Cells through Aberrant Autophagy
Figure 5
The knockdown of Beclin 1 decreases GNA-induced cancer cell death.
A, RNA oligonucleotides against Beclin 1 (Beclin 1 siRNA) effectively repress Beclin 1 expression. A549 cells were transfected with siRNAs against Beclin 1 or negative control, and the protein level of Beclin1 was analyzed by western blotting. GAPDH protein was used as the loading control. The bar graph shows the band intensities of Beclin1 relative to those of GAPDH. Mean± SEM, n = 3, ** p<0.01, one-way ANOVA. B, A549 cells were transfected with siRNAs against Beclin 1 or negative control. The cells were then treated with 3 µM GNA for the indicated periods of time, stained with 2 µM Hoechst 33342 and observed under a fluorescent microscope; the percentage of dead cells was quantified by Trypan blue staining. Mean± SEM, n = 3, *means p<0.05, ** p<0.01, one-way ANOVA.