Rosamines Targeting the Cancer Oxidative Phosphorylation Pathway
Figure 5
Inhibition of mitochondrial oxidative phosphorylation complexes.
The dose-response inhibition of mitochondrial oxidative phosphorylation Complex 1 (A), Complex II (B), Complex IV (C) and ATP synthase (D) activities by rosamine 2 (solid line) and 5 (dotted line). The activity of Complex II was partially inhibited by 5 with IC50 value of 9.6±0.1 µM whereas for 2, inhibition was observed but with undetermined IC50 value. Both 2 and 5 also inhibited the ATP synthase activities with IC50 values of 3.9±0.3 and 3.0±0.8 µM respectively. The activity of Complex I and Complex IV were not affected by the rosamines at the treated concentrations (highest at 10 µM). IC50 values depict concentration that inhibits the complexes activity by 50%. ND - indicate non-determined IC50 values based on the concentration used.