Role of IGF-Binding Protein 3 in the Resistance of EGFR Mutant Lung Cancer Cells to EGFR-Tyrosine Kinase Inhibitors
Figure 2
Expression of EGFR-related signals in HCC827 and resistant cell lines.
(A) Basal expression of EGFR and EGFR-related signalling molecules in HCC827, HCC827/GR and HCC827/ER cells were evaluated by Western blotting. The effects of gefitinib (B) and erlotinib (C) on EGFR-related signalling were also examined. HCC827, HCC827/GR and HCC827/ER cells were treated with 0.1 and 1 µM gefitinib and erlotinib for 72 h in medium containing 1% FBS. Protein (30 µg) from cell lysates was subjected to Western blot analysis for the indicated proteins.