The Campylobacter jejuni Cj0268c Protein Is Required for Adhesion and Invasion In Vitro
Figure 5
Flow cytometric measurements of permeabilized and non-permeabilized E. coli cells expressing Cj0268c (pRRC-cj0268c) or transformed with an empty vector (pRRC).
Bacteria were immunolabelled with a monoclonal anti-pentaHis primary antibody and a R-phycoerythrin-conjugated secondary antibody. E. coli cells of both populations were gated according to the forward scatter and the side scatter (dot plots, left panel). Permeabilized E. coli cells harbouring the empty plasmid pRRC without gene cj0268c (upper panel) possess a considerable lower fluorescence intensity as compared to an E. coli population which express Cj0268c (lower panel). To verify the specificity of binding of anti-pentaHis, a control staining with the secondary antibody only was included (right panel).