The Campylobacter jejuni Cj0268c Protein Is Required for Adhesion and Invasion In Vitro
Figure 4
Detection of Cj0268c in E. coli and altered adherence.
1. E. coli DH5α/pRRC, 2. E. coli DH5α/pRRC-cj0268cHis. (a) Immunoblot with a monoclonal antibody against the His-tag detected a protein with a molecular weight of 41 kDa corresponding to Cj0268c. The protein was exclusively found in the lysate of the Cj0268c-expressing E. coli. The respective Coomassie staining served as a loading control. (b) Adherence assays on Caco2 cells with Cj0268c-expressing E. coli and E. coli transformed with plasmid pRRC alone. Taken the number of recovered E. coli colonies from the strain harbouring pRRC without cj0268c as 100%, the mean value of the adherence capacity of E. coli DH5α/pRRC-cj0268cHis was 201.7% (±6.44, P<0.0039).