The Campylobacter jejuni Cj0268c Protein Is Required for Adhesion and Invasion In Vitro
Figure 1
Generation of a cj0268c-knockout mutant and a complemented mutant in C. jejuni strain NCTC 11168.
(1) parental strain NCTC 11168, (2) cj0268c knockout mutant (NCTC 11168::cj0268c), (3) complemented knockout mutant (NCTC 11168::cj0268c-comp-cj0268c). (a) Genome arrangements of the bacterial strains under investigation. Primers Cj0268cF and Cj0268cR for the amplification ofcj0268c are indicated by arrowheads. The primer KanF that binds to the 5′-end of the kanamycin resistance cassette is shown by an arrow. (b) Verification of the native gene, the respective mutant and the complemented mutant strain by PCR. (1) PCR analysis with Cj0268cF + Cj0268cR-primers detect the native gene of 1090 bp. (2) aphA-3 insertion in gene cj0268c mediating kanamycin resistance in mutant strain NCTC 11168::cj0268c was verified by the amplification of a 2914 bp PCR- amplicon applying primers Cj0268cF+R. (3) the complemented mutant was verified by PCR analysis with primers Cj0268cF+R to detect the native gene (1090 bp) and with primers KanF + Cj0268cR which amplify a PCR product of 2168 bp.