A Protocol for Quantifying Lipid Peroxidation in Cellular Systems by F2-Isoprostane Analysis
Figure 1
The chemical structure and mass spectrum of F2-isoprostanes.
(A) Four isomeric classes of F2-isoprostanes are formed as a result of oxidative damage to arachidonic acid. (B) The product ion scan of F2-class-III and F2-class-VI isoprostanes. The ion masses indicated in red were used as MRM transition pairs for the indicated isoprostanes.