Novel Histopathological and Molecular Effects of Natural Compound Pellitorine on Larval Midgut Epithelium and Anal Gills of Aedes aegypti
Figure 9
Gene expression patterns by pellitorine treatment.
(A) RT-PCR of AaAQP4, AaV-H+-ATPase and Aarps7 genes in control and treatment groups. (B) Relative gene expression level of AaV-type H+-ATPase and aquaporin 4 (AaAQP4), compared to the expression level of Aarps7 gene. The gene expression of AaV-type H+-ATPase and aquaporin 4 (AaAQP4) in the anal gills was inhibited in a larva treated with 5 mg/l natural pellitorine as compared to the control larvae. * indicates significant difference in gene expression levels in control and pellitorine treated groups (Student's t-test, N = 3, P<0.01).