Novel Histopathological and Molecular Effects of Natural Compound Pellitorine on Larval Midgut Epithelium and Anal Gills of Aedes aegypti
Figure 3
Histology of thorax and midgut regions of third instar Ae. aegypti larvae.
(A) Control mosquito. A1. anterior midgut region of control larvae has well-developed gastric caeca (GC) and single-layered midgut epithelium. A2. Central midgut region of control larvae consisted of well-developed lumen content (LC), peritrophic space (PS), and peritrophic membrane (PM). A3. Posterior midgut region of control larvae consisted of distinguished midgut epithelial layer, lumen contents, and peritrophic membrane. (B) Treated mosquito with 5 mg/l natural pellitorine showed undistinguished enlarged portion of gastric caeca and damaged single-layered epithelial cells. B1. Pellitorine-treated larvae had an undistinguished enlarged portion of gastric caeca and damaged single-layered epithelial cells (asterisks). B2. Central midgut region of pellitorine-treated larvae showed demolished epithelial layer residues mixed with a few LC (asterisks). B3. Complete damaged residue of epithelial and peritrophic membranes was observed in pellitorine-treated larvae (asterisk).