Osteoblast CFTR Inactivation Reduces Differentiation and Osteoprotegerin Expression in a Mouse Model of Cystic Fibrosis-Related Bone Disease
Figure 2
Lack of CFTR expression in osteoclasts.
(A) Cftr mRNA expression analysis of calvarial osteoblasts, undifferentiated and osteoclast-differentiated (M-CSF/RANKL-treated) RAW264.7 murine monocyte cells. Trap mRNA expression served as a control for osteoclast formation. (B) Immunofluorescence did not reveal CFTR (red) expression in murine bone marrow-derived multinucleated osteoclasts. Membrane-localized protein Na+/H+ exchange regulatory cofactor-1 (NHERF1) (green) served as a positive control for membrane staining. Nuclei (blue) was identified by DAPI staining.