Proteasomal Degradation of Mcl-1 by Maritoclax Induces Apoptosis and Enhances the Efficacy of ABT-737 in Melanoma Cells
Figure 2
Down regulation of Mcl-1 induces apoptosis in melanoma cells.
Melanoma cells were incubated with either indicated amount of Maritoclax for 24h to access dose response or with 5 µM Maritoclax for mentioned time points. After incubation, cells were harvested; total lysates were prepared, and fractionated. The Western blot analysis was performed using mentioned antibodies and same blots were re-probed with either other antibodies or with anti-GAPDH to access loading. The results shown are representative of two independent experiments. A-B, Maritoclax had no effect on Bcl-2 and Bcl-xL. C-D, Degradation of Mcl-1 is associated with apoptosis.