TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression
Figure 2
TIMP-1 promotes in vivo growth of prostate cancers.
A. Establishment of pooled populations of PC3, 22RV1, and LAPC-4 cells expressing v5 epitope tagged TIMP-1 or transduced with the empty expression vector alone (controls). Secreted v5-tagged TIMP-1 was detected by anti-v5 mAb (Invitrogen). B. TIMP-1 promotes PC3 and 22RV1 prostate cancer growth in vivo. Weights of the tumors derived from PC3 and 22RV1 prostate cancer cells expressing TIMP-1v5 or infected with the empty expression constructs (PC3-control and 22RV1-control) were measured 5 or 10-weeks, respectively, after subcutaneous implantation of the cancer cells into Rag-2/II2rg mice (Taconic). n=6. *p<0.05. C-E. Growth rates of the subcutaneous prostate tumors derived from PC3 (C), 22RV1 (D), and LAPC-4 (E) cells expressing TIMP-1v5 or transduced with the empty expression vector (controls) as indicated in the panels. The growth rates are expressed as the mean of tumor volumes (mm3) +/- SD. Six mice were used for each type of transduced prostate cells. *p<0.05.