Effect of Iboga Alkaloids on µ-Opioid Receptor-Coupled G Protein Activation
Figure 5
Effect of ibogaine and 18-MC on [35S]GTPγS binding in Sprague-Dawley rat thalamic membranes compared with DAMGO (Reith lab).
Tissue suspension aliquots were incubated with indicated drug and 0.09 nM of [35S]GTPγS for 1 h at 30°C. Data are expressed as % of maximal stimulation by 10 µM DAMGO and presented as mean ± SEM (vertical bar) for 3 - 4 independent experiments assayed in triplicate.