The Fumagillin Gene Cluster, an Example of Hundreds of Genes under veA Control in Aspergillus fumigatus
Figure 7
Expression of fumR and PKS370 is regulated by laeA.
Total RNA was extracted from WT and ∆laeA strains, grown as stationary liquid Czapek-Dox cultures for 48 h and 72 h. Relative expression of fumR and PKS gene Afu8g00370 was calculated using 2-ΔΔCt method as described by Schmittgen and Livak [89]. Primers used for the expression analysis are listed in Table S2. The bar represents the mean of three replicates and error bars represent standard error. Expression of 18S was used as internal reference. Values were normalized to expression levels of wild-type levels considered as 1.