The Fumagillin Gene Cluster, an Example of Hundreds of Genes under veA Control in Aspergillus fumigatus
Figure 6
fumR regulates fumagillin biosynthesis.
Aspergillus fumigatus wild type (WT TSD51.1) and ∆fumR strains were grown on Czapek-Dox as stationary liquid cultures for 72 h and 120 h. Culture supernatants were extracted with chloroform and analyzed by LC-MS (A) as described in materials and methods. (B) Quantitative data of the LC-MS analysis. The experiment was done with three replicates. Error bars represent standard error. No fumagillin was detected (n.d.) in ∆fumR mutants. A standard curve was obtained using commercial fumagillin (Sigma).