The Fumagillin Gene Cluster, an Example of Hundreds of Genes under veA Control in Aspergillus fumigatus
Figure 5
fumR is necessary for the expression of the genes in the fumagillin cluster.
The transcriptional pattern of the genes in the fumagillin cluster (Afu8g00370, Afu8g00520, Afu8g00370, Afu8g00510, Afu8g00500, Afu8g00480, Afu8g00470, Afu8g00440, Afu8g00430) was evaluated by qRT-PCR in the wild type (WT, TSD51.1) and αfumR strains. Total RNA was extracted from Czapek-Dox stationary liquid cultures incubated for 48 h and 72 h. The relative expression was calculated using 2-ΔΔCT as described by Schmittgen and Livak [89]. 18S gene expression was used as internal reference. Means of three replicates is shown. Values were normalized to WT expression at 48 h considered as 1. Error bar represents standard error.