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The Fumagillin Gene Cluster, an Example of Hundreds of Genes under veA Control in Aspergillus fumigatus

Figure 4

veA regulates the production of fumagillin, fumitremorgin G, fumigaclavine C and Glionitrin A.

Secondary metabolites were extracted from 120 h old Czapek-dox stationary liquid cultures of wild type (WT), ΔveA, complementation and OEveA strains. Extracts were analyzed with Shimadzu 2010 EV LC-MS as described in the materials and methods section. The predicted m/z [M+H]+ ratio was (A) fumagillin (m/z=459), (B) fumitremorgin G (m/z = 433) (C) fumigaclavine C (m/z = 299) and (D) Glionitrin-A (m/z = 354). The bars represent the mean of three samples and error bars represent standard error.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0077147.g004