Type V Collagen Induced Tolerance Suppresses Collagen Deposition, TGF-β and Associated Transcripts in Pulmonary Fibrosis
Figure 5
Tolerance induction of col(V) suppresses T lymphocytes activation and associated pro-inflammatory/pro-fibrotic cytokine expression.
(A) At day 21 post bleomycin injury, mediastinal lymphocytes were isolated and cultured alone or in the presence of autologous antigen presenting cells (APCs from naïve C57-BL/6 mice) for 48 h. The cells were then radiolabeled with tritiated thymidine for 16 h and assessed for proliferation rates. Values represent mean ± SEM. n = 5 mice/group; Compared to bleomycin group, * p<0.01; one-way ANOVA, post hoc test: Bonferroni. (B) Conditioned media from A was analyzed for Th1/Th2/Th17 cytokines. Values represent mean ± SEM. n = 5 mice/group; Compared to bleomycin group, * p<0.01; one-way ANOVA, post hoc test: Bonferroni. (C, D) Plasma samples from (A) were analyzed for Th1/Th2/Th17 cytokines. Values represent mean ± SEM. n = 5 mice/group; Compared to bleomycin group, * p<0.01; one-way ANOVA, post hoc test: Bonferroni. (E) At day 14 post bleomycin injury, whole lung homogenates from bleomycin-injured mice+col(V) treatment were quantified for il-17a mRNA expression and normalized for β-actin. Values represent mean ± SEM. n = 5 mice/group; compared to bleomycin group, * p<0.01; one-way ANOVA, post hoc test: Bonferroni.