REEPs Are Membrane Shaping Adapter Proteins That Modulate Specific G Protein-Coupled Receptor Trafficking by Affecting ER Cargo Capacity
Figure 6
REEP expression in non-permeabilized HEK293A, NRK, and Rat1 cells.
HEK293A, NRK, and Rat1 cells were transfected with Flag-REEP1, -REEP2, or –REEP6. Forty-eight hrs later, cells were fixed with 4% PFA, but not permeabilized, for immunofluorescent labeling. Flag-REEPs were labeled with M2 (anti-Flag) antisera and identified with Alexa 594-conjugated goat anti-mouse secondary antibody. Note the similar strong peri-nuclear and intracellular reticular staining pattern seen in all three cell lines. Representative of three separate transfections. Scale bars: 10 µm.