Utilisation of Mucin Glycans by the Human Gut Symbiont Ruminococcus gnavus Is Strain-Dependent
Figure 5
Relative level of transcription of R. gnavus ATCC 29149 nan genes.
Fold change in gene transcription has been determined by qRT-PCR for the nan genes when R. gnavus ATCC 29149 was grown in presence of pPGM (white box) or 3′SL (grey box) compared to Glc as sole carbon source. The results showed averages of two biological replicates, each performed in 3 technical replicates. Data were analysed using 1-way ANOVA. For each gene, a post-hoc test (Dunnett's) was used to examine if there were any significant differences in each condition (versus Glc). The transcription of nanH was significantly increased when R. gnavus ATCC 29149 was grown with either pPGM or 3′SL compared to Glc. The transcription of both nanK and nanA was also significantly increased when ATCC 29149 was grown with 3′SL compared to Glc. *: p<0.05; **, p<0.01.