NMK-TD-100, a Novel Microtubule Modulating Agent, Blocks Mitosis and Induces Apoptosis in HeLa Cells by Binding to Tubulin
Figure 5
Effect of NMK-TD-100 on interphase and spindle microtubules in HeLa cells.
(A-I) NMK-TD-100 perturbed the organization of interphase microtubule in HeLa cells. Cells were incubated in various concentration of NMK-TD-100 (0-5 µM) for 24 h, fixed and stained using antibody against α-tubulin (red) and nucleus with DAPI (blue). (J-O) NMK-TD-100 depolymerized the spindle microtubule with formation of multipolar spindles in HeLa cells. Microtubules were stained with antibody against α-tubulin (red) and nucleus with DAPI (blue). Details of the experiments are described in the ‘Materials and methods’ section.