Cell-Intrinsic Role for NF-kappa B-Inducing Kinase in Peripheral Maintenance but Not Thymic Development of Foxp3+ Regulatory T Cells in Mice
Figure 6
NIK is required cell-intrinsically for normal proportions of memory phenotype conventional T cells.
Single cell suspensions of nucleated splenocytes from intact (A-E), single BM chimeric (F-J), or mixed BM chimeric (K-O) mice were stained with the indicated fluorescently labeled antibodies. Dot plots are gated on CD4+ (A), CD4+CD45.1- (F), or CD4+CD45.1-CD45.2+ (K) cells. Numbers on plots indicate percent of gated cells. B, G, and L, Quantitation of percent Foxp3-CD44hi within the gated populations described in A, F, and K, respectively. C, H, and M, Quantitation of absolute number of CD4+ memory T cells of the indicated genotype per spleen. D, I, and N, Quantitation of absolute number of CD4+ naive T cells of the indicated genotype per spleen. E, J, and O, Quantitation of naïve: memory CD4+ T cell ratio of the indicated genotype. Data in bar graphs represent mean ± SEM of n=3-4 mice per group in one representative experiment of two (A-E and K-O) or three (F-J).