Cell-Intrinsic Role for NF-kappa B-Inducing Kinase in Peripheral Maintenance but Not Thymic Development of Foxp3+ Regulatory T Cells in Mice
Figure 3
Decreased peripheral Foxp3+ Tregs in both intact NIK KO and NIK KO single BM chimeric mice.
Single cell suspensions of nucleated splenocytes from intact (A-F) or BM chimeric (G-L) mice were stained with the indicated fluorescently labeled antibodies. A, Plots are gated on CD4+; numbers indicate percent of gated cells. B and E, Quantitation of percent of Foxp3+ and CD4+ splenocytes, respectively. C, D, and F, Quantitation of absolute number of Foxp3+, total, and CD4+ splenocytes. G, Plots are gated on CD4+CD45.1- cells (BM-derived, NIK KO or WT littermate). Numbers indicate percent of gated cells. H and K, Quantitation of percent of Foxp3 + CD45.1- and CD4+CD45.1- cells, respectively. I, J, and L, Quantitation of absolute number of Foxp3 + CD45.1-, total CD45.1-, and CD4+CD45.1- splenocytes, respectively. Data in bar graphs represent mean ± SEM of n=3-4 mice per group in one representative experiment of two (B-F) or three (G-L).